SIRT7 promotes lung cancer progression by destabilizing the tumor suppressor ARF

Sirtuin 7 (SIRT7) is a member of the mammalian family of nicotinamide adenine dinucleotide (NAD(+))-dependent histone/protein deacetylases, known as sirtuins. It acts as a potent oncogene in numerous malignancies, but the molecular mechanisms employed by SIRT7 to sustain lung cancer progression remain largely uncharacterized. We demonstrate that SIRT7 exerts oncogenic functions in lung cancer cells by destabilizing the tumor suppressor alternative reading frame (ARF). SIRT7 directly interacts with ARF and prevents binding of ARF to nucleophosmin, thereby promoting proteasomal-dependent degradation of ARF. We show that SIRT7-mediated degradation of ARF increases expression of protumorigenic genes and stimulates proliferation of non-small-cell lung cancer (NSCLC) cells both in vitro and in vivo in a mouse xenograft model. Bioinformatics analysis of transcriptome data from human lung adenocarcinomas revealed a correlation between SIRT7 expression and increased activity of genes normally repressed by ARF. We propose that disruption of SIRT7-ARF signaling stabilizes ARF and thus attenuates cancer cell proliferation, offering a strategy to mitigate NSCLC progression.

  • Kumari, P.
  • Tarighi, S.
  • Fuchshuber, E.
  • Li, L.
  • Fernández-Duran, I.
  • Wang, M.
  • Ayoson, J.
  • Castelló-García, J. M.
  • Gámez-García, A.
  • Espinosa-Alcantud, M.
  • Sreenivasan, K.
  • Guenther, S.
  • Olivella, M.
  • Savai, R.
  • Yue, S.
  • Vaquero, A.
  • Braun, T.
  • Ianni, A.

Keywords

  • Humans
  • *Sirtuins/metabolism/genetics
  • *Lung Neoplasms/genetics/metabolism/pathology
  • Animals
  • Mice
  • *Carcinoma, Non-Small-Cell Lung/genetics/metabolism/pathology
  • *Cell Proliferation
  • *Disease Progression
  • Gene Expression Regulation, Neoplastic
  • Cell Line, Tumor
  • Arf
  • Sirt7
  • Sirtuins
  • lung cancer
  • nucleophosmin
Publication details
DOI: 10.1073/pnas.2409269121
Journal: Proc Natl Acad Sci U S A
Pages: e2409269121
Number: 25
Work Type: Original
Access number: 38870055
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