Long noncoding RNAs (lncRNA) are crucial yet underexplored regulators of human immunity. Here we develop GRADR, a method integrating gradient profiling with RNA-binding proteome analysis, to map the protein interactomes of all expressed RNAs in a single experiment to study mechanisms of lncRNA-mediated regulation of human primary macrophages. Applying GRADR alongside CRISPR-multiomics, we reveal a network of NF
- Schmerer, N.
- Janga, H.
- Aillaud, M.
- Hoffmann, J.
- Aznaourova, M.
- Wende, S.
- Steding, H.
- Halder, L. D.
- Uhl, M.
- Boldt, F.
- Stiewe, T.
- Nist, A.
- Jerrentrup, L.
- Kirschbaum, A.
- Ruppert, C.
- Rossbach, O.
- Ntini, E.
- Marsico, A.
- Valasarajan, C.
- Backofen, R.
- Linne, U.
- Pullamsetti, S. S.
- Schmeck, B.
- Schulte, L. N.
Keywords
- Humans
- *RNA, Long Noncoding/genetics/metabolism
- *Macrophages/metabolism/immunology
- *Gene Regulatory Networks
- GATA2 Transcription Factor/genetics/metabolism
- NF-kappa B/metabolism
- Macrophage Activation/genetics
- Gene Expression Regulation
- CRISPR-Cas Systems
