Tumor-associated macrophages (TAMs) possess both tumor-promoting and tumor-inhibiting roles. Here, we explore TAMs' anti-tumor functions, focusing on the immune responsive gene 1 (IRG1) and its product, itaconate, in lung cancer development. Spatial metabolomics reveals that endogenous itaconate is markedly depleted within lung tumor regions compared with adjacent non-tumor tissue. Single-cell RNA sequencing shows that macrophages are the primary cells expressing IRG1 in human and mouse lung tumors. Both IRG1 knockout and transplantation of IRG1-depleted bone marrow leads to increased lung tumor growth in various mouse lung tumor models. Additionally, 4-octyl itaconate (Octyl Ita) reduces tumor growth in vitro, in vivo, and in ex vivo human tumor precision-cut lung slices. An integrated multi-omics analysis shows that IRG1/itaconate causes a metabolic shift in cancer cell and pro-tumor macrophages, mainly by inhibiting the pentose phosphate pathway (PPP) through targeting glucose-6-phosphate dehydrogenase (G6PD) activity, thereby suppressing cancer cell growth and transforming pro-tumor macrophages into anti-tumor macrophages. Thus, leveraging IRG1/itaconate's tumor-suppressive effects or using Octyl Ita could be a novel lung cancer therapy.
- Mansouri, S.
- Hesami, G.
- Ambikan, A.
- Karger, A.
- Klatt, S.
- Neogi, U.
- Kurakula, K. B.
- Aliraj, B.
- Miller, A.
- Petrova, B.
- Sanda, M.
- Sirait-Fischer, E.
- Guenther, S.
- Kuenne, C.
- Ruppert, C.
- Alkoudmani, I.
- Gattenlohner, S.
- Zukunft, S.
- Fleming, I.
- Haschemi, A.
- Stiewe, T.
- Grimminger, F.
- Reck, M.
- Weigert, A.
- Seeger, W.
- Pullamsetti, S. S.
- Savai, R.
Keywords
- glucose-6-phosphate dehydrogenase
- itaconate
- lung cancer
- tumor-associated macrophages
